Table of ContentsGeneral Methods.Progress in Antisense Technology.Basic Prinicples of Using Antisense Oligonucleotides in Vivo.Polyethyleneimine-Mediated Transfection to Improve Antisense Activity of 3'-Capped Phosphodiester Oligonucleotides.Design of Antisense and Triplex-Forming Oligonuceotides.Chimeric Oligodeoxynucleotide Analogs.Evaluation of Antisense Mechanisms of Action.Physiology and Molecular Biology Brought to Single-Cell Level.Antisense Properties of Peptide Nucleic Acid.Synthesis of Oligonucleotide Conjugates in Anhydrous Dimethyl Sulfoxide.Gene Switching.Vesicular Stomatitis Virus as Model System for Studies of Antisense Oligonucleotide Translation Arrest.Purification of Antisense Oligonuceleotides.Boranophosphate Backbone.Intracellular Distribution of Digoxigenin-Labled Phosphorothioate Oligonucleotides.Use of Minimally Modified Antisense Oligonucleotides for Specific Inhibition of Gene Expression.