This is the third volume in the Methods in Cell Biology series to cover protein translocation within and between cells. Volumes 31 and 32, Vesicular Transport Parts A and B, treated techniques for eukaryotic cells available as of 1989. This text brings together new methods and topics as of 1991. Highlights include use of anti-idiotype antibodies, gene fusions, transcription, crosslinking, pulse-labeling and reconstitution.