Cochran (periodontics, University of Texas Health Science Center) explains principles for the clinical use of enamel matrix derivative (EMD) in periodontal regeneration in this manual for general practitioners, clinicians working in periodontal regeneration, and students studying biology-based regenerative treatment approaches. After an overview of trends in periodontal regeneration and the biological basis for the use of EMD, the manual addresses aspects of patient selection, mechanisms of action, and treatment of various conditions, with step-by-step procedures illustrated by color photos and drawings and b&w X-rays.